Analysis of Ala aminoacyl synthetase.

Analysis performed on 2024-09-30 10:19:47

Number of Proteins Analyzed

Initial Filtered Out Final Blast
Bacteria 45892 712 45180 26362
Archaea 3414 132 3282 1408
Vertebrata 1592 72 1520 529
Mammals 595 27 568 138

Number of Variants Analyzed:

Protein Length Pathogenic Variants Benign/Unknown Variants
968 89 985

Domains

Domains
Current Study No Domains Found
Guo et al (2010) No Domains Listed

Results

Mutations in Ancient vs Modern Domains

Current Study

Ancient Domains Modern Domains All Ratio
Number of Residues 968 0 968 Undefined
Number of Variants 1074 0 1074 Undefined
Number of Pathogenic Variants 89 0 89 Undefined
Number of Benign/Unknown Variants 985 0 985 Undefined
Fisher exact test Undefined

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Guo et al (2010)

Ancient Domains Modern Domains All Ratio
Number of Residues 968 0 968 Undefined
Number of Variants 1074 0 1074 Undefined
Number of Pathogenic Variants 89 0 89 Undefined
Number of Benign/Unknown Variants 985 0 985 Undefined
Fisher exact test Undefined

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Coverage of the alignment and domain locations

Agreement between the current study and Guo et al (2010)

Conservation of the amino acids in modern and ancient domains

# of sites Ancient # sites Modern Mean Ancient (SD) Mean Modern (SD) Median Ancient Median Modern Mann-Whitney U p-value
Current Study 968 0 0.77 (0.01) nan (nan) 7.615e-01 NaN Undefined Undefined
Guo et al (2010) 968 0 0.77 (0.01) nan (nan) 7.615e-01 NaN Undefined Undefined

The Mann-Whitney U test was calculated using the conservation scores of the amino acids in ancient and modern domains. The p-value is for the null hypothesis that the conservation scores in ancient domains are greater than in modern domains.

Conservation of the amino acids in modern and ancient domains